The shutters came down for the final time at Tesco in Morecambe’s Arndale on Saturday.
The decision to close the centre’s flagship store, which employed 45 people, was made earlier this year after owners said it was unprofitable.
It was one of 43 stores to go as the retail chain culled shops across the country.
The new Arndale owners moved to quash fears for the shopping centre’s future after Tesco announced its closure plans in January.
New River Retail, who bought the Arndale in a £14m deal just before Christmas, said they still planned to invest “considerable funds” in the centre, despite the loss of Tesco.
A New River retail spokesman said: “We remain confident in Morecambe following our recent investment and are already engaged with the BID (Business Improvement District), Town Team and council in helping to drive regeneration of the town centre and a wider master plan.
“We see this as a sustainable impact investment with positive social benefits.
“Tesco was not the reason we chose to invest in the town.”
As the Morecambe supermarket shut up shop for the last time at 6pm on Saturday, staff posed for a picture to mark the occasion.